Home

Quick change mutagenesis manual

The QuikChange II sitedirected mutagenesis kit is used to make point mutations, replace amino acids, and delete or insert single or multiple adjacent amino acids. b The QuikChange II XL SiteDirected Mutagenesis Kit (Catalog# ) contains enough reagents for 10 total reactions, which includes 5 control reactions.

c Thaw the dNTP mix once, prepare singleuse aliquots, and store the aliquots at 20C. a The QuikChange SiteDirected Mutagenesis Kit (Catalog# ) contains enough reagents for 30 total reactions, which includes 5 control reactions. b The QuikChange SiteDirected Mutagenesis Kit (Catalog# ) contains enough reagents for 10 total reactions, which includes 5 control reactions.

c See Preparation of Media and Reagents. Directed Mutagenesis Kit is a proprietary Pfubased polymerase blend and the newly optimized Dpn I enzyme, which together allow for mutagenesis in approximately one hour, plus an overnight transformation. FIGURE 1 Overview of the QuikChange Lightning sitedirected mutagenesis method. a The QuikChange SiteDirected Mutagenesis Kits contain enough reagents for 5 control reactions. b See Preparation of Media and Reagents.

c Thaw the dNTP mix once, prepare singleuse aliquots, and store the aliquots at 20C. QuikChange Multi SiteDirected Mutagenesis Kit Instruction Manual Catalog# and# Revision D Research Use Only.

Not for Use in Diagnostic Procedures. Figure 2: Q5 SiteDirected Mutagenesis Kit Overview. This kit is designed for rapid and efficient incorporation of insertions, deletions and substitutions into doublestranded plasmid DNA. The first step is an exponential amplification using standard primers and a master mix fomulation of Q5 Hot Start HighFidelity DNA Polymerase. In vitro sitedirected mutagenesis is an invaluable technique for studying gene and protein structurefunction relationships and for modifying vector sequences to facilitate cloning and expression strategies.

QuikChange II SiteDirected Mutagenesis Kit 3 The QuikChange II sitedirected mutagenesis kit is used to make point mutations, replace amino acids, and delete or insert single or multiple QuikChange SiteDirected Mutagenesis Kit Instruction Manual Catalog# (30 reactions) and (10 reactions) Revision C Research Use Only.

a The QuikChange SiteDirected Mutagenesis Kit (Catalog# ) contains enough reagents for 30 total reactions, which includes 5 control reactions. b The QuikChange SiteDirected Mutagenesis Kit (Catalog# ) contains enough reagents for 10 total reactions, which includes 5 control reactions.

c See Preparation of Media and Reagents.